Reporter

Part:BBa_I712051:Design

Designed by: Anja Korenčič   Group: iGEM07_Ljubljana   (2007-10-22)


pT7-rLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 599


Design Notes

Part is cloned into pSBAK3 vector with terminator. Luciferase gene includes start and stop codon.


Source

Source for renilla luciferase part was biobrick BBa_J52008 (made by iGEM contestants). T7 promoter comes from T7 bacteriophage.

References